Seminars in Spine Surgery
Volume 18, Issue 1 , Pages 2-11 , March 2006

Does Autologous Bone Marrow Aspirate Enhance Graft Effectiveness for Spinal Fusion?

  • Hyun Bae, MD

      Affiliations

    • Spine Research Foundation and Spine Institute at Saint John’s Health Center, Santa Monica, CA
    • Corresponding Author InformationAddress reprint requests to Hyun Bae, MD, Spine Research Foundation, 1301 20th Street, Suite 400, Santa Monica, CA 90404
  • ,
  • L.E.A. Kanim, MA

      Affiliations

    • Preclinical Science, Spine Research Foundation, Santa Monica, CA
  • ,
  • Erik Spayde, MD

      Affiliations

    • Spine Institute at Saint John’s Health Center, Santa Monica, CA
  • ,
  • Pamela Wong, BS

      Affiliations

    • Preclinical Science, Spine Research Foundation, Santa Monica, CA
  • ,
  • Li Zhao, MD, PhD

      Affiliations

    • Preclinical Science, Spine Research Foundation, Santa Monica, CA
  • ,
  • Ben Pradhan, MD, MSE

      Affiliations

    • Spine Research Foundation and Spine Institute at Saint John’s Health Center, Santa Monica, CA
  • ,
  • Rick Delamarter, MD

      Affiliations

    • Spine Research Foundation and Spine Institute at Saint John’s Health Center, Santa Monica, CA

References 

  1. Goujon E . Recherches expérimentales sur les propriétés physiologiques de la moelle des os . J de l’ Anatomie et de la Physiologie Normales et Pathologiques de l’ Homme et des Animaux . 1869;6:399
  2. Burwell RG . A study of homologous cancellous bone combined with autologous red marrow after transplantation to a muscular site . J Anat . 1961;95:613
  3. Green E , Hinton C , Triffitt JT . The effect of decalcified bone matrix on the osteogenic potential of bone marrow . Clin Orthop Relat Res . 1986;205:292–298
  4. Lindholm T , Urist M . A quantitative analysis of new bone formation by induction in composite grafts of bone marrow and bone matrix . Clin Orthop . 1980;150:288–300
  5. Burwell RG . The function of bone marrow in the incorporation of a bone graft . Clin Orthop Relat Res . 1985;200:125–141
  6. Owen M , Friedenstein AJ . Stromal stem cells (marrow-derived osteogenic precursors) . Ciba Found Symp . 1988;136:42–60
  7. Beresford JN . Osteogenic stem cells and the stromal system of bone and marrow . Clin Orthop . 1989;240:270–280
  8. Caplan AI . Mesenchymal stem cells (cell-based reconstructive therapy in Orthopedics) . Tissue Eng . 2005;11(7-8:1198–1211
  9. Salama R . Xenogeneic bone grafting in humans . Clin Orthop Relat Res . 1983;174:113–121
  10. Wientroub S , Goodwin D , Khermosh O , et al.   The clinical use of autologous marrow to improve osteogenic potential of bone grafts in pediatric orthopedics . J Pediatr Orthop . 1989;9(2):186–190
  11. Cavagna R , Daculsi G , Bouler JM . Macroporous calcium phosphate ceramic (a prospective study of 106 cases in lumbar spinal fusion) . J Long Term Eff Med Implants . 1999;9(4):403–412
  12. Papavero L , Zwonitzer R , Burkard I , et al.   A composite bone graft substitute for anterior cervical fusion (assessment of osseointegration by quantitative computed tomography) . Spine . 2002;27(10):1037–1043
  13. Price CT , Connolly JF , Carantzas AC , et al.   Comparison of bone grafts for posterior spinal fusion in adolescent idiopathic scoliosis . Spine . 2003;28(8):793–798
  14. Korovessis P , Koureas G , Zacharatos S , et al.   Correlative radiological, self-assessment and clonical analysis of evolution in instrumented dorsal and lateral fusion for degenerative lumbar spine disease. Autograft versus coralline hydroxyapatite . Eur Spine J . 2005;14:630–638
  15. Kitchel SH . A prospective study of mineralized collagen and bone marrow aspirate as a bone graft substitute in instrumented posterolateral lumbar fusion 1 and 2 year follow-up. Proc NASS 20th Annual Meeting . Spine J . 2005;5:95S
  16. Boden SD , Kang J , Sandhu H , et al.   Use of recombinant human bone morphogenetic protein-2 to achieve posterolateral lumbar spine fusion in humans: a prospective, randomized clinical pilot trial: 2002 Volvo Award in clinical studies . Spine . 2002;27(23):2662–2673
  17. Vaccaro AR , Patel T , Fischgrund J , et al.   A pilot study evaluating the safety and efficacy of OP-1 Putty (rhBMP-7) as a replacement for iliac crest autograft in posterolateral lumbar arthrodesis for degenerative spondylolisthesis . Spine . 2004;29(17):1885–1892
  18. Caplan AI . Mesenchymal stem cells . J Orthop Res . 1991;9(5):641–650
  19. Caplan AI . The mesengenic process . Clin Plast Surg . 1994;21(3):429–435
  20. Muschler GF , Boehm CA , Easley KA . Aspiration to obtain osteoblast progenitor cells from human bone marrow (the influence of aspiration volume) . J Bone Joint Surg Am . 1997;79:1699–1709
  21. Block JE . The role and effectiveness of bone marrow in osseous regeneration . Med Hypotheses . 2005;65(4):740–747
  22. Kitchel SH , Wang MY , Lauryssen CL . Techniques for aspirating bone marrow for use in spinal surgery . Neurosurgery . 2005;57(suppl 4):286–289
  23. D’Ippolito G , Schiller PC , Ricordi C . Age-related osteogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow . J Bone Miner Res . 1999;14:1115–1122
  24. Cinotti G , Patti AM , Vulcano A , et al.   Experimental posterolateral spinal fusion with porous ceramics and mesenchymal stem cells . J Bone Joint Surg Br . 2004;86(1):135–142 Erratum in J Bone Joint Surg Br 86(4):622, 2004
  25. Minamide A , Yoshida M , Kawakami M , et al.   The use of cultured bone marrow cells in type I collagen gel and porous hydroxyapatite for posterolateral lumbar spine fusion . Spine . 2005;30(10):1134–1138
  26. Andrades JA , Han B , Becerra J , et al.   A recombinant human TGF-beta1 fusion protein with collagen-binding domain promotes migration, growth, and differentiation of bone marrow mesenchymal cells . Exp Cell Res . 1999;250(2):485–498
  27. Glazer PA , Spencer UM , Alkalay RN , et al.   In vivo evaluation of calcium sulfate as a bone graft substitute for lumbar spinal fusion . Spine J . 2001;1(6):395–401
  28. Hartman EH , Vehof JW , Spauwen PH , et al.   Ectopic bone formation in rats (the importance of the carrier) . Biomaterials . 2005;26(14):1829–1835
  29. Curylo LJ , Johnstone G , Petersilge CA , et al.   Augmentation of spinal arthrodesis with autologous bone marrow in a rabbit posterolateral spine fusion model . Spine . 1999;24(5):434–438
  30. Shih HN , Shih LY , Sung TH , et al.   Restoration of bone defect and enhancement of bone ingrowth using partially demineralized bone matrix and marrow stromal cells . J Orthop Res . 2005;23(6):1293–1299
  31. Muschler GF , Matsukura Y , Nitto H , et al.   Selective retention of bone marrow-derived cells to enhance spinal fusion . Clin Orthop Relat Res . 2005;432:242–251
  32. Lindholm TS , Nilsson OS , Lindholm TC . Extraskeletal and intraskeletal new bone formation induced by demineralized bone matrix combined with bone marrow cells . Clin Orthop Relat Res . 1982;171:251–255
  33. Ragni P , Lindholm TS , Lindholm TC . Vertebral fusion dynamics in the thoracic and lumbar spine induced by allogenic demineralized bone matrix combined with autogenous bone marrow . Ital J Orthop Traumatol . 1987;13:241–252
  34. Lindholm TS , Ragni P , Lindholm TC . Response of bone marrow stroma cells to demineralized cortical bone matrix in experimental spinal fusion in rabbits . Clin Orthop Relat Res . 1988;230:296–302
  35. Ragni P , Ala-Mononen P , Lindholm TS . Spinal fusion induced by porous hydroxyapatite blocks (HA). Experimental comparative study with HA, demineralized bone matrix and autogenous bone marrow . Ital J Orthop Traumatol . 1993;19(1):133–144
  36. Wang JC , Kanim LE , Yoo S , et al.   Effect of regional gene therapy with bone morphogenetic protein-2-producing bone marrow cells on spinal fusion in rats . J Bone Joint Surg Am . 2003;85A(5):905–911 Erratum in J Bone Joint Surg Am 85A(9):1801, 2003
  37. Harris CT , Cooper LF . Comparison of bone graft matrices for human mesenchymal stem cell-directed osteogenesis . J Biomed Mater Res . 2004;68A:747–755
  38. Gazdag AR , Lane JM , Glaser D , et al.   Alternatives to autogenous bone graft (efficacy and indications) . J Am Acad Orthop Surg . 1995;3(1):1–8
  39. Kim H , Suh H , Sangmee AJ , et al.   In vivo bone formation by human marrow stromal cells in biodegradable scaffolds that release dexamethasone and ascorbate-2-phosphate . Biochem Biophys Res Commun . 2005;332:1053–1060
  40. van Gaalen SM , Dhert WJ , van den Muysenberg A , et al.   Bone tissue engineering for spine fusion (an experimental study on ectopic and orthotopic implants in rats) . Tissue Eng . 2004;10(1-2):231–239
  41. Cui Q , Ming Xiao Z , Balian G , et al.   Comparison of lumbar spine fusion using mixed and cloned marrow cells . Spine . 2001;26(21):2305–2310
  42. Tay BK , Le AX , Heilman M , et al.   Use of a collagen-hydroxyapatite matrix in spinal fusion. A rabbit model . Spine . 1998;23(21):2276–2281
  43. Walsh WR , Loefler A , Nicklin S , et al.   Spinal fusion using an autologous growth factor gel and a porous resorbable ceramic . Eur Spine J . 2004;13(4):359–366
  44. Boden SD , Martin GJ , Morone M , et al.   The use of coralline hydroxyapatite with bone marrow, autogenous bone graft, or osteoinductive bone protein extract for posterolateral lumbar spine fusion . Spine . 1999;24(4):320–327
  45. Kraiwattanapong C , Boden SD , Louis-Ugbo J , et al.   Comparison of Healos/bone marrow to INFUSE (rhBMP-2/ACS) with a collagen-ceramic sponge bulking agent as graft substitutes for lumbar spine fusion . Spine . 2005;30(9):1001–1007 discussion 1007
  46. Bareille R , Lafage-Proust MH , Faucheux C , et al.   Various evaluation techniques of newly formed bone in porous hydroxyapatite loaded with human bone marrow cells implanted in an extra-osseous site . Biomaterials . 2000;21(13):1345–1352
  47. Callewart CC , Kanim LEA , Seeger LL , et al.   Variable fusion rates in the canine model . Orthop Trans . 1995;(19):601
  48. Kai T , Shao-qing G , Geng-ting D . In vivo evaluation of bone marrow stromal-derived osteoblasts-porous calcium phosphate ceramic composites as bone graft substitute for lumbar intervertebral spinal fusion . Spine . 2003;28(15):1653–1658
  49. Walsh WR , Harrison J , Loefler A , et al.   Mechanical and histologic evaluation of Collagraft in an ovine lumbar fusion model . Clin Orthop Relat Res . 2000;375:258–266
  50. Wang T , Dang G , Guo Z , et al.   Evaluation of autologous bone marrow mesenchymal stem cell-calcium phosphate ceramic composite for lumbar fusion in rhesus monkey interbody fusion model . Tissue Eng . 2005;11(7-8):1159–1167
  51. Orii H , Sotome S , Chen J , et al.   Beta-tricalcium phosphate (beta-TCP) graft combined with bone marrow stromal cells (MSCs) for posterolateral spine fusion . J Med Dent Sci . 2005;52(1):51–57
  52. Bae HW , Zhao L , Kanim L , et al.   Bone marrow enhances the performance of rhBMP-2 . Cervical Spine Res Soc . 2005;33:32
  53. Liu XD , Zhu YK , Umino T , et al.   Cigarette smoke inhibits osteogenic differentiation and proliferation of human osteoprogenitor cells in monolayer and three-dimensional collagen gel culture . J Lab Clin Med . 2001;137(3):208–219
  54. Delecrin J , Deschamps C , Romih M , et al.   Influence of bone environment on ceramic osteointegration in spinal fusion (comparison of bone-poor and bone-rich sites) . Eur Spine J . 2001;2(suppl 2):S110–S113
  55. Gurevitch O , Kurkalli BG , Prigozhina T , et al.   Reconstruction of cartilage, bone, and hematopoietic microenvironment with demineralized bone matrix and bone marrow cells . Stem Cells . 2003;21(5):588–597

PII: S1040-7383(06)00003-7

doi: 10.1053/j.semss.2006.01.002

Seminars in Spine Surgery
Volume 18, Issue 1 , Pages 2-11 , March 2006